Elisabeth of Culemborg (30 March 1475, the former slot of Hoogstraten - 9 December 1555, Culemborg), nicknamed 'Lady Elizabeth ('Vrouwe Elisabeth') was the last lord or lady of the fiefdom of Culemborg (promoted to a county by Charles V, Holy Roman Emperor shortly before her death). She was the rich heiress of jonker Jasper of Culemborg and Joanna of Burgundy, rising to chief lady-in-waiting to her niece Archduchess Margaret of Austria.
In 1491 Elisabeth married Jan van Luxemburg, who died in 1508. She remarried in 1509 to Antoon I van Lalaing, later acting-governor of the Netherlands.